Aqueous Two Phase Partitioning

Author: Boris Y. Zaslavsky
Publisher: CRC Press
ISBN: 9780824794613
Size: 25.50 MB
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Aqueous Two Phase Partitioning from the Author: Boris Y. Zaslavsky. Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Partitioning In Aqueous Two Phase System

Author: Harry Walter
Publisher: Elsevier
ISBN: 0323142915
Size: 41.13 MB
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Partitioning In Aqueous Two Phase System from the Author: Harry Walter. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two Phase Systems

Author: Rajni Hatti-Kaul
Publisher: Springer Science & Business Media
ISBN: 1592590284
Size: 20.49 MB
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Aqueous Two Phase Systems from the Author: Rajni Hatti-Kaul. In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products

Author: Marco Rito-Palomares
Publisher: Springer
ISBN: 3319593099
Size: 58.62 MB
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Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products from the Author: Marco Rito-Palomares. This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

International Conference On Partitioning In Aqueous Two Phase Systems Advances In Separation In Biochenistry Cell Biology And Biotechnology 7th Held In New Orleans Louisiana On 2 7 June 1991

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ISBN:
Size: 51.65 MB
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International Conference On Partitioning In Aqueous Two Phase Systems Advances In Separation In Biochenistry Cell Biology And Biotechnology 7th Held In New Orleans Louisiana On 2 7 June 1991 from the Author: . This is the detailed report and proceedings from The 7th International Conference on Partitioning in Aqueous Tw6-Phase Systems: Advances in Separation in Biochemistry Cell Biology and Biotechnology, June 2-7, 1991, New Orleans, Louisiana, U.S.A.